Photocage-Selective Capture and Light-Controlled Release of Target Proteins

Photocage-Selective Capture and Light-Controlled Release of Target Proteins

Blog Article

Summary: Photochemical transformations enable exquisite spatiotemporal control over biochemical processes; however, methods for reliable manipulations of biomolecules tagged with biocompatible photo-sensitive reporters are lacking.Here we created a high-affinity binder specific to a photolytically removable caging group.We utilized chemical modification or genetically encoded incorporation of noncanonical amino acids to produce proteins with photocaged cysteine or selenocysteine residues, moen s73004srs which were used for raising a high-affinity monoclonal antibody against a china glaze beaches and toes small photoremovable tag, 4,5-dimethoxy-2-nitrobenzyl (DMNB) group.Employing the produced photocage-selective binder, we demonstrate selective detection and immunoprecipitation of a variety of DMNB-caged target proteins in complex biological mixtures.This combined orthogonal strategy permits photocage-selective capture and light-controlled traceless release of target proteins for a myriad of applications in nanoscale assays.